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Literature summary extracted from

  • Rausch, J.W.; Chelico, L.; Goodman, M.F.; Le Grice, S.F.
    Dissecting APOBEC3G substrate specificity by nucleoside analog interference (2009), J. Biol. Chem., 284, 7047-7058.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.B9 expressed in Sf9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.B9 D316R/D317R the mutations increase affinity for substrate and deamination specificity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.B9 Zn2+ contains zinc Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.B9 46000
-
 x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O Homo sapiens the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently uracil in single-stranded viral DNA + NH3
-
 ?
3.5.4.B9 additional information Homo sapiens the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.B9 glutathione-Sepharose resin column chromatography and DEAE FF column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 5'-AAAGAGAAAGAGAAACCCAAAGAGGAAAGGTGAGGAGAA-3' + H2O the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently Homo sapiens 5'-AAAGAGAAAGAGAAACCUAAAGAGGAAAGGTGAGGAGAA-3' + NH3
-
 ?
3.5.4.B9 cytosine in single-stranded viral DNA + H2O the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently Homo sapiens uracil in single-stranded viral DNA + NH3
-
 ?
3.5.4.B9 additional information the enzyme does not effectively bind substrates shorter than 10 nucleotides. Substrates containing 5'-methyldeoxycytidine 2'-deoxy-5-aza-5,6-dihydrocytidine, 2'-deoxy-4-ethylcytidine and 2'-deoxyzebularine at position -1 are deaminated by the enzyme with 62%, 25%, 19%, and 9% efficiency, respectively, Substrates containing N3-methyl cytidine or isocytidine at position -1 are not appreciably deaminated by the enzyme Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.B9 monomer or dimer x * 46000, the enzyme is active in monomeric, dimeric, and larger oligomeric states Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 A3G
-
 Homo sapiens
3.5.4.B9 APOBEC3G
-
 Homo sapiens
3.5.4.B9 apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G
-
 Homo sapiens